Beckman Coulter Navios EX Flow Cytometer

Lasers: 

Blue Solid State: 488 nm, 55 mW, Software Controlled

Red Solid State Diode: 638 nm, 50 mW, Software Controlled

Violet Solid State: 405 nm, 80 mW, Software Controlled

Configuration: Nominal 90 µm spatially separated beam spots

Flow cell: 430 µm x 180 µm rectangular channel

Collection optics: Fixed integrated optics and quartz flow cell design with a numerical aperture of > 1.2

Detector filters: 

Forward Scatter: 488/20

Blue Laser: 525/40, 575/30, 614/20 , 675/20, 695/30, 755LP

Dyes: FITC, PE, ECD, PC5 or PEC5.5, PECy7

Red Laser: 660/20, 725/20, 755 LP

Dyes: APC or Alexa Fluor 647, APC-Alexa Fluor 700, APC-Cy7, APC-Alexa Fluor 750

Violet Laser: 450/50, 550/40

Dyes: Pacific Blue, Krome Orange

Forward Scatter Detector: Fourier design providing up to 3 measurements of forward angle

Side Scatter Detector: High performance photodiode with electronic attenuation

Fluorescence Detectors: FL1 – FL6 Fluorescent Detectors

Flow rates: Continuous pressure is applied to the sample tube based on user selected flow rates: Low, Medium and High

Performance: Carryover: < 0.1%, Dead volume as low as 2 µL when using Beckman Coulter 12 x 75 mm polypropylene tube and adjustable probe

Acquisition modes: 32 tube Multi Carousel Loader (MCL), Single tube sampling mode, Automated work list acquisition, Manual work list mode

Mixing: The MCL vortexes each tube individually before sample acquisition

Barcode reading: Carousel number, tube location, and tube barcode verified to worklist

Biosafety: Biohazard contained wash station thoroughly rinses sample probe

Throughput: 10000 normal leukocytes is 82 tubes/hour

Scatter resolution: Resolves 0.4 micron particles from background noise using Forward Scatter, Maximum detection of up to 40 microns 

Fluorescence sensitivity: FITC < 107 MESF, PE < 64 MESF, PC5 < 13 MESF

Acquisition rate: 25000 events per second, 90% yield

Cytometer dimensions (WxHxD): 950 x 610 x 730 mm

Cytometer weight: 104 kg 

Supply cart dimensions (WxHxD): 724 x 298 x 495 mm

Supply cart weight: 30 kg

Software included but requires a new licence purchase.