BD Becton Dickinson FACSCanto II Flow Cytometer

The FACSCanto II system is an easy-to-use benchtop analyzer that delivers proven performance, accuracy, and high-quality results. The FACSCanto II can be configured with two or three lasers to detect up to eight colors. It features many innovations, including a true fixed alignment flow cell to minimize startup time and improve  reproducibility. The optical system maximizes signal detection and increases sensitivity and resolution for each color in a multicolor assay. These and other capabilities make the BD FACSCanto II ideal for today’s busy clinical lab, providing a high degree of automation and quality control. With optimal reproducibility, the FACSCanto II system reduces hands-on technician time and costs associated with repeat testing.

Lasers:

405-nm solid state diode, 30-mW fiber power output

488-nm solid state, 20-mW laser output

633-nm HeNe, 17-mW laser output

Laser Configuration: Spatially separated beams with 9 x 65-

μm elliptical spots

Optical Alignment Procedure: Fixed, no operator alignment required

Flow Cell: 180 x 430-μm rectangular quartz flow cell

Collection Optics: Optical-gel coupled 1.2 NA lens

FSC Resolution: 1.0 μm

SSC Resolution: 0.5 μm

Fluorescence Detector Design: Reflective optics with single transmission filter in front of each PMT

FSC Detector: Photodiode with 488/10 BP

SSC Detector: PMT with 488/10 BP

Fluorescence Detectors: 8 PMTs in 4-2-2 configuration

Violet Laser Dyes: Pacific Blue, AmCyan (455, 488 nm)

Blue Laser Dyes: FITC, PE, PerCP or PerCP-Cy 5.5, PE-Cy 7 (525, 575, 678 or 695, 785 nm)

Red Laser Dyes: APC, APC-Cy7 (660, 785 nm)

Detector Bands:

Violet Laser: 450/50; 502 to 525 nm

Blue Laser: 530/30; 585/42; >670; 780/60 nm

Red Laser: 660/20; 780/60 nm

Fluorescence Threshold Sensitivities: FITC <100 MESF; PE <50 MESF

Max Particle Size: 50 μm

Sample Flow Rate: 10 μL/min to 120 μL/min

Sample Acquisition Rate: 10,000 events/second, 6 compensated

fluorescence parameters and 2 scatter parameters

Sample Dead Volume: 30 μL (BD Falcon tubes 12 x 75-mm)

Dimensions (DxWxH): 

Cytometer: 610 x 910 x 640 mm

Fluidics cart: 610 x 790 x 640 mm

Weight:

Cytometer: 145 kg

Fluidics cart: 51 kg