Bio-Rad Gene Pulser Xcell Electroporation System

The Gene Pulser Xcell System is a flexible, modular electroporation system for transfecting every cell type from primary, suspension, and difficult-to-transfect cells, including T cells, to bacteria and fungi. The system is composed of a main unit, two accessory modules, the capacitance extender (CE module) and the pulse controller (PC module), and a ShockPod cuvette chamber.

Features and Benefits

Universal electroporation — transfects all cell types, from primary, stem, and T cells to bacteria and fungi

Flexibility with your experiment parameters for the highest transfection efficiency and viability

Preset protocols — ready-to-use preoptimized protocols for frequently used mammalian and bacterial cell types

Manual operation — allows for entry and storage of new parameters or editing of all parameters

Optimization protocols — enables the best conditions to be determined using incremental voltage steps

Exponential and square waveforms — allows for optimal delivery

Confidence in your results — uses PulseTrac circuitry and arc-protection features to ensure sample protection and reproducible results

Protocol library — collection of electroporation protocols for every cell type including primary, immortal, and bacterial cells

Prevalidated published protocols — saves time optimizing conditions

Easily accessed data management — storage and recall of parameters and results from previous 100 experiments, useful for troubleshooting

Output Waveform: Exponential or square

Output Voltage: 10 – 3,000 V

Capacitance: 10 – 500 V, 25 – 3,275 µF in 25 µF increments, 500 – 3,000 V, 10, 25, 50 µF

Resistance (parallel): 50 – 1,000 Ω in 50 Ω increments, plus infinity

Sample resistance: 20 Ω minimum at 10 – 2,500 V, 600 Ω minimum at 2,500 – 3,000 V

Square-wave timing: 10 – 500 V: 0.05 – 10 ms in 0.05 ms increments, 10 – 100 ms pulse in 1 ms increments, 1 – 10 pulses, 0.1 – 10 sec interval/ 500 – 3,000 V: 0.05 – 5 ms in 0.05 ms increments, 1 – 2 pulses, 5 sec minimum interval

Dimensions (WxDxH): 310 x 300 x 280 mm

Weight: 11.6 kg