GE Healthcare ÄKTApurifier UPC10 System

The GE AKTA Purifier 10 FPLC System is versatile, modular liquid chromatography systems for fast and reliable separations of proteins, peptides, and nucleic acids by FPLC. AKTA Purifier 10 is designed for purification of microgram to milligram quantities of protein, operating at flow rates of 0.001 to 10 ml/min at pressures of 0 to 25 MPa.

Flow Rate Range:

Isocratic mode: 0.01 to 100 ml/min in steps of 10 µl/min

Gradient mode: 0.01 to 100 ml/min in steps of 10 µl/min

Double mode: 0.01 to 200 ml/min in steps of 10 µl/min

Pressure Range: 0 to 10 MPa (100 bar, 1450 psi)

pH Stability Range: 1 to 13 (1 to 14 with <1 day exposure)

Viscosity: Max 5 cP

Flow Rate Accuracy (0.2 – 10 MPa): ± 2% or 20 µl/min, whichever is greater

Flow Rate Reproducibility (flow rate ≥ 0.5 ml/min): rsd < 0.5%

Gradient Composition Accuracy: < ±1% at 0.5 to 100 ml/min

Gradient Composition Reproducibility: rsd < 0.25% at 0.5 to 100 ml/min

Pressure Sensor Range: 0 to 27.5 MPa

Offset error: < 0.05 MPa

Scale error: < ±2%

Total Volume Between Inlet and Outlet: < 800 µl/module

Wavelength range: 190 – 700 nm in steps of 1nm, 3 wavelenghts simultaneously

Bandwidth: 4 nm

Wavelength accuracy: ±2 nm

Wavelength reproducibility: ±0.01 nm

Wavelength switch time: < 500 ms (one cycle from 214 nm to 254 nm and back to 214 nm)

Linearity: <2 % deviation up to 2 AU at 260 nm with Uracil at pH 2

Noise:

Single wavelength (short term, 0.5 – 1 min): <6×10⁻⁵ AU at 230 nm

Single wavelength (long therm, 1 – 10 min): <6×10⁻⁵ AU at 230 nm

Dual wavelength (short term, 0.5 – 1 min): <2×10⁻⁴ AU at 230 nm and 254 nm

Dual wavelength (long therm, 1 – 10 min): <2×10⁻⁴ AU at 230 nm and 254 mm

Drift: <2×10⁻⁴ AU at 254 mm

System consists of:

UPC-900 monitor

P-900 pump

P-960 pump

2x INV-907 valve

2x PV-908 valve
FRAC-950 fraction collector

PC